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dc.contributor.author Tavares, Lucas Alves
dc.contributor.author Rodrigues, Roger Luiz
dc.contributor.author Santos da Costa, Cristina
dc.contributor.author Nascimento, Jonas Alburqueque
dc.contributor.author Vargas de Carvalho, Julianne
dc.contributor.author Nogueira de Carvalho, Andreia
dc.contributor.author Mardones, Gonzalo A.
dc.contributor.author daSilva, Luis L.P.
dc.date.accessioned 2024-09-12T03:43:01Z
dc.date.available 2024-09-12T03:43:01Z
dc.date.issued 2024-03
dc.identifier.issn 0021-9258
dc.identifier.uri https://repositorio.uss.cl/handle/uss/11639
dc.description Publisher Copyright: © 2024 The Authors
dc.description.abstract Selective retrograde transport from endosomes back to the trans-Golgi network (TGN) is important for maintaining protein homeostasis, recycling receptors, and returning molecules that were transported to the wrong compartments. Two important transmembrane proteins directed to this pathway are the Cation-Independent Mannose-6-phosphate receptor (CI-MPR) and the ATP7B copper transporter. Among CI-MPR functions is the delivery of acid hydrolases to lysosomes, while ATP7B facilitates the transport of cytosolic copper ions into organelles or the extracellular space. Precise subcellular localization of CI-MPR and ATP7B is essential for the proper functioning of these proteins. This study shows that both CI-MPR and ATP7B interact with a variant of the clathrin adaptor 1 (AP-1) complex that contains a specific isoform of the γ-adaptin subunit called γ2. Through synchronized anterograde trafficking and cell-surface uptake assays, we demonstrated that AP-1γ2 is dispensable for ATP7B and CI-MPR exit from the TGN while being critically required for ATP7B and CI-MPR retrieval from endosomes to the TGN. Moreover, AP-1γ2 depletion leads to the retention of endocytosed CI-MPR in endosomes enriched in retromer complex subunits. These data underscore the importance of AP-1γ2 as a key component in the sorting and trafficking machinery of CI-MPR and ATP7B, highlighting its essential role in the transport of proteins from endosomes. en
dc.language.iso eng
dc.relation.ispartof vol. 300 Issue: no. 3 Pages:
dc.source Journal of Biological Chemistry
dc.title AP-1γ2 is an adaptor protein 1 variant required for endosome-to-Golgi trafficking of the mannose-6-P receptor (CI-MPR) and ATP7B copper transporter en
dc.type Artículo
dc.identifier.doi 10.1016/j.jbc.2024.105700
dc.publisher.department Facultad de Medicina y Ciencia


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