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dc.contributor.author Hidalgo, Cecilia
dc.contributor.author Aracena, Paula
dc.contributor.author Sanchez, Gina
dc.contributor.author Donoso, Paulina
dc.date.accessioned 2024-09-26T00:44:02Z
dc.date.available 2024-09-26T00:44:02Z
dc.date.issued 2002
dc.identifier.issn 0716-9760
dc.identifier.uri https://repositorio.uss.cl/handle/uss/13305
dc.description.abstract In skeletal and cardiac muscle cells, specific isoforms of the Ryanodine receptor channels mediate Ca2+ release from the sarcoplasmic reticulum. These channels are highly susceptible to redox modifications, which regulate channel activity. In this work, we studied the effects of Ca2+ (endogenous agonist) and Mg2+ (endogenous inhibitor) on the kinetics of Ca2+ release from sarcoplasmic reticulum vesicles isolated from skeletal or cardiac mammalian muscle. Native skeletal vesicles exhibited maximal stimulation of release kinetics by 10-20 μM [Ca2+], whereas in native cardiac vesicles, maximal stimulation of release required only 1 μM [Ca2+]. In 10 μM [Ca2+], free [Mg2+] < 0.1 mM produced marked inhibition of release from skeletal vesicles but free [Mg2+] ≤ 0.8 mM did not affect release from cardiac vesicles. Incubation of skeletal or cardiac vesicles with the oxidant thimerosal increased their susceptibility to stimulation by Ca2+ and decreased the inhibitory effect of Mg2+ in skeletal vesicles. Sulfhydryl-reducing agents fully reversed the effects of thimerosal. The endogenous redox species, glutathione disulfide and S-nitrosoglutathione, also stimulated release from skeletal sarcoplasmic reticulum vesicles. In 10 μM [Ca2+], 35S-nitrosoglutathione labeled a protein fraction enriched in release channels through S-glutathiolation. Free [Mg2+] 1 mM or decreasing free [Ca2+] to the nM range prevented this reaction. Possible physiological and pathological consequences of redox modification of release channels on Ca2+ signaling in heart and muscle cells are discussed. en
dc.language.iso eng
dc.relation.ispartof vol. 35 Issue: no. 2 Pages: 183-193
dc.source Biological Research
dc.title Redox regulation of calcium release in skeletal and cardiac muscle en
dc.type Artículo
dc.identifier.doi 10.4067/S0716-97602002000200009
dc.publisher.department Facultad de Medicina y Ciencia


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