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dc.contributor.author Troncoso, Felipe
dc.contributor.author Acurio, Jesenia
dc.contributor.author Herlitz, Kurt
dc.contributor.author Aguayo, Claudio
dc.contributor.author Bertoglia, Patricio
dc.contributor.author Guzman-Gutierrez, Enrique
dc.contributor.author Loyola, Marco
dc.contributor.author Gonzalez, Marcelo
dc.contributor.author Rezgaoui, Meriem
dc.contributor.author Desoye, Gernot
dc.contributor.author Escudero, Carlos
dc.date.accessioned 2024-09-26T00:46:54Z
dc.date.available 2024-09-26T00:46:54Z
dc.date.issued 2017-08
dc.identifier.issn 1932-6203
dc.identifier.uri https://repositorio.uss.cl/handle/uss/13508
dc.description Publisher Copyright: © 2017 Troncoso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstract Placentas from gestational diabetes mellitus (GDM) are often hypervascularized; however, participation of vascular endothelial growth factor (VEGF) and its receptors in this placental adaptation is unclear. We aimed to test whether changes in phosphorylation of tyrosine 951 or tyrosine 1175 (pY951 or pY1175) of the vascular endothelial growth factor receptor 2 (KDR) are associated with the proangiogenic state observed in placentas from GDM. We obtained placental samples from women with normal pregnancies (n = 24) or GDM (n = 18). We measured the relative expression of markers for endothelial cell number (CD31, CD34), VEGF, vascular endothelial growth factor receptor 1 (Flt-1), KDR, pY951 and pY1175 of KDR in placental homogenate. Immunohistochemistry of placental blood vessels were performed using CD34. Proliferation and migration of human umbilical vein endothelial cells (HUVEC) obtained from normal pregnancy and GDM were determined in absence or presence of conditioned medium (CM) harvested from GDM or normoglycemic HUVEC cultures. GDM was associated with more CD31 and CD34 protein compared to normal pregnancy. High number, but reduced area of placental blood vessels was found in GDM. Reduced Flt-1 levels (mRNA and protein) are associated with reduced KDR mRNA, but higher KDR protein levels in placentas from GDM. No significant changes in Y951-or Y1175-phosphorylation of KDR in placentas from GDM were found. GDM did not alter proliferation of HUVECs, but enhanced migration. Conditioned medium harvested from GDM HUVEC cultures enhanced KDR protein amount, tube formation capacity and cell migration in HUVEC isolated from normoglycemic pregnancies. The data indicate that GDM is associated with reduced expression of Flt-1 but high pro-migratory activation of KDR reflecting a proangiogenic state in GDM. en
dc.language.iso eng
dc.relation.ispartof vol. 12 Issue: no. 8 Pages:
dc.source PLoS ONE
dc.title Gestational diabetes mellitus is associated with increased pro-migratory activation of vascular endothelial growth factor receptor 2 and reduced expression of vascular endothelial growth factor receptor 1 en
dc.type Artículo
dc.identifier.doi 10.1371/journal.pone.0182509
dc.publisher.department Facultad de Medicina y Ciencia
dc.publisher.department Facultad de Ciencias de la Naturaleza
dc.publisher.department Facultad de Medicina Veterinaria


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